Faculty

Dr. Neelam Azad Dr. Neelam Azad Associate Professor and Chairperson, Pharmaceutical Sciences Location:Flemmie Kittrell Hall1st Floor, Main Office Phone:757-727-5071

Dr. Neelam Azad joined the School of Pharmacy at Hampton University in August 2008 and was promoted to the rank of Associate Professor and the position of department Chairperson in 2014. Dr. Azad’s vision is to provide high quality education in pharmaceutical sciences and to maintain a vigorous biomedical sciences research program. As part of her scholarly duties, Dr. Azad coordinates and teaches Physiological Chemistry (PHA 311) and Medicinal Chemistry (PHA 314). Dr. Azad’s research interests are in the field of lung pathologies and cancer cell biology, and she has published extensively in a number of high-profile scientific journals. Dr. Azad has received multiple NIH-funded SCORE grant awards and a recently funded sub-award under Hampton University’s U54 grant award. She serves as co-investigator on a training (R25) grant aimed at establishing a multi-year summer research-training program for pharmacy professional students in areas pertaining to lung pathologies.

 

Education

Ph.D. (Pharmaceutical Sciences), West Virginia University, 2007

B.S. (Pharmacy), University of Mumbai, 2001

 

Honors & Awards

2016                    Academic Leadership Fellows Program (ALFP) Certification, American Association of Colleges of Pharmacy

2015, 2014, 2013 Professor of the Year Award, Department of Pharmaceutical Sciences, Hampton University

2014, 2013, 2012 AACR Minority-Serving Institution Faculty Scholar Award, America Association for Cancer Research Annual Conference

2013                    President’s Ambassador for Academic Excellence Award,Hampton University

2013                    Scholarship, Frontiers in Stem Cells in Cancer Workshop, Magee Women’s Research Institute, University of Pittsburgh

2012                    Scholarship, Workshop on Leadership in Bioscience, Cold Spring Harbor Laboratory

2010                    Induction into Rho Chi Honor Society

2010                    Conference Award, Towards Personalized Cancer Medicine Conference, The New York Academy of Sciences

2006                    Research Award in Biotechnology, American Association of Pharmaceutical Scientists Annual Conference

2006                    Induction into Sigma Xi Honor Society

2006                    Sigma Xi Research Award, Chapter’s Annual Research Day, West Virginia University

2006                    Annual Research Award, Department of Pharmaceutical and Pharmacological Sciences, West Virginia University

2006, 2005          Van Liere Research Award,Health Science Center’s Annual Research Day, West Virginia University

2004                    Best Teaching Assistant Award, Department of Pharmaceutical and Pharmacological Sciences, West Virginia University


Grantsmanship

ONGOING RESEARCH SUPPORT

9SC1GM121287-06A1                                                                              06/26/2018 - 05/31/2022

Minority Biomedical Research Support – Support for Continuous Research Excellence (MBRS-SCORE)

National Institute of Health (NIH)/National Institute of General Medical Sciences

Title: Targeting Lipogenic and Angiogenic Mediators in Pulmonary Fibrosis

Role: Principal Investigator            Amount: $ 1.27 M

 

SC3GM122655

MBRS – SCORE                                                                                09/11/2017 - 08/31/2021

National Institute of Health (NIH)/ National Institute for General Medical Sciences (NIGMS)

Title: Reversing Resistance to PARP-Inhibitor in Triple Negative Breast Cancer Cells

Role: Co-investigator                              Amount: $ 444,000

 

R25HL125453                                                                                   09/01/2016 - 06/30/2021

NIH/ National Heart Lung and Blood Institute (NHLBI)

Title: The Summer Pharmacy Experiences in Academic Research program at Hampton University.

Role: Co-investigator                             Amount: $ 427,799

 

SC1CA173069-01A1                                                                         09/01/2012 - 08/31/2018

NIH/National Cancer Institute (NCI)

Title: Anti-Tumorigenic Potential of Novel Digitoxin Analogues

Role: Co-investigator                               Amount: $ 1.35 M

 

U54MD008621

The Hampton University Regional Transdisciplinary Collaborative Center

National Institute of Health (NIH)

Sub Award number: HU-180004                                                     10/01/2017 - 06/30/2018

Title: Role of Wnt/β-catenin in Prostate Cancer Progression in Obese African-Americans

Role: Co-investigator                               Amount: $ 93,750

 

COMPLETED RESEARCH SUPPORT

 

SC1HL112630-01                                                                              09/01/2011 - 06/30/2017

Minority Biomedical Research Support – Support for Continuous Research Excellence (MBRS-SCORE)

National Institute of Health (NIH)/National Heart Lung and Blood Institute

Title: Impact of Oxidative Stress-Mediated Angiogenesis in Pulmonary Fibrosis

Role: Principal Investigator            Amount: $ 1.3 M

 

Faculty Development Grant                                                             09/01/2010 - 12/31/2011

Hampton University

Title: Anti-Tumorigenic Effects of Novel Digitoxin Analogues

Role: Co-investigator                     

 

Faculty Development Grant                                                             09/01/2010 - 12/31/2011

Hampton University

Title: Impact of Oxidative Stress-Mediated Angiogenesis in Pulmonary Fibrosis

Role: Principal investigator             

 

Faculty Development Grant                                                              01/01/2010 - 12/31/2010

Hampton University

Title: Elucidation of Novel Signaling Mechanisms in Carbon Nanotube Induced Carcinogenesis

Role: Co-investigator                     

 

Research Development Grant                                                          01/01/2008 - 01/01/2009

West Virginia University

Title: Role of Akt/PKB in Mediating Carbon Nanotube-Induced Pathogenesis

Role: Co-investigator   

 

Research Interests

  • Fibrosis – Genetic and molecular networks involved in pulmonary fibrosis.
  • Cancer – Molecular mechanisms involved in cancer progression and metastasis.
  • Apoptosis – Effect of environmental and occupational carcinogens on apoptosis.
  • Free Radical Biology – Oxidative and nitrosative stress in cancer and fibrosis.

 

Publications

       1.          Kulkarni YM, Dutta S, Iyer AKV, Wright CA, Ramesh V, Kaushik V, Semmes OJ, Azad N. A Lipidomics Approach to Identifying Key Lipid Species Involved in VEGF-Inhibitor Mediated Attenuation of Bleomycin-Induced Pulmonary Fibrosis. Proteomics Clinical Applications (2017). [Epub ahead of print]

       2.          Kaushik V., Yakisich J.S., Azad N., Iyer A.K.V. Alternative Models of Cancer Stem Cells: Implications for Translational Oncology. Frontiers in Stem Cell and Regenerative Medicine Research (2017), Volume 8, 1-54.

       3.          Yakisich J.S., Kulkarni Y., Azad N., Iyer A.K.V. Selective and Irreversible Induction of Necroptotic Cell Death in Lung Tumorspheres by Short-Term Exposure to Verapamil in Combination with Sorafenib. Stem Cells International (2017). [Epub ahead of print]

       4.          Guishard A.F., Yakisich J.S., Azad N., Iyer A.K. Translational gap in ongoing clinical trials for glioma. Journal of Clinical Neuroscience (2017), S0967-5868(17), 31048-31052.

       5.          Azad N., Anderson H.G. Jr., Brooks A., Garza O., O'Neil C., Stutz M.M., Sobotka J.L.   Leadership and Management Are One and the Same. The American Journal of Pharmaceutical Education (2017), 81(6):102.

       6.          Kulkarni Y.M., Yakisich J.S., Azad N., Venkatadri R., Kaushik V., O'Doherty G., Iyer A.K.V. Anti-tumorigenic effects of a novel digitoxin derivative on both estrogen receptor-positive and triple-negative breast cancer cells. Tumour Biology (2017), 39(6).

       7.          Venkatadri R., Iyer A.K.V., Kaushik V., Azad N. A novel resveratrol-salinomycin combination sensitizes ER-positive breast cancer cells to apoptosis. Pharmacological Reports (2017), 69(4):788-797.

       8.          Wright C, Iyer AKV, Kaushik V, Azad N. Anti-Tumorigenic Potential of a Novel Orlistat-AICAR Combination in Prostate Cancer Cells. Journal of Cellular Biochemistry (2017). [Epub ahead of print]

       9.          Yakisich J.S., Azad N., Kaushik V., O'Doherty G.A., Iyer A.K. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biology (2017), 9(3).

    10.          Wright C., Iyer A.K., Yakisich J.S., Azad N. Anti-Tumorigenic Effects of Resveratrol in Lung Cancer Cells Through Modulation of c-FLIP. Current Cancer Drug Targets. (2017). [Epub ahead of print]

    11.          Kaushik V., Azad N., Yakisich J.S., Iyer AK. Antitumor effects of naturally occurring cardiac glycosides convallatoxin and peruvoside on human ER+ and triple-negative breast cancers. Cell Death Discovery (2017), 3:17009

    12.          Yakisich J.S., Azad N., Kaushik V., Iyer A.K.V. Cancer Cell Plasticity: Rapid Reversal of Chemosensitivity and Expression of Stemness Markers in Lung and Breast Cancer Tumorspheres. Journal of Cellular Physiology (2017), 232(9):2280-2286.

    13.          Kaushik V., Yakisich J.S, Azad N., Kulkarni Y., Venkatadri R., Wright C., Rojanasakul Y., Iyer A.K.V. Anti-Tumor Effects of Cardiac Glycosides on Human Lung Cancer Cells and Lung Tumorspheres. Journal of Cellular Physiology (2017), 232(9):2497-2507.

    14.         Venkatadri R., Iyer A.K., Ramesh V., Wright C., Castro C.A., Yakisich J.S., Azad N. MnTBAP Inhibits Bleomycin-Induced Pulmonary Fibrosis by Regulating VEGF and Wnt Signaling. Journal of Cellular Physiology (2017), 232(3):506-516.

    15.         Yakisich J.S., Venkatadri R., Azad N., Iyer A.K.V. Chemoresistance of Lung and Breast Cancer Cells Growing Under Prolonged Periods of Serum Starvation. Journal of Cellular Physiology (2017), 232(8):2033-2043. doi: 10.1002/jcp.25514. Epub 2017 Feb 28.

    16.         Wright C. A., Iyer A.K.V, Wang L., Wu N., Yakisich J.S., Rojanasakul Y., Azad N.. Effects of titanium dioxide nanoparticles on human keratinocytes. Drug Chem Toxicol. (2016), 16, 1-11. 

    17.         Venkatadri R., Muni T., Iyer A.K.V., Yakisich J.S., Azad N..Role of Apoptosis-Related MiRNAs in Resveratrol-Induced Breast Cancer Cell Death.  Cell Death and Disease (2016). (Epub ahead of print)

    18.         Klionsky D.J., Azad N. et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy (2016), 12 (1): 1-222.

    19.         Yakisich J.S., Azad N., Venkatadri R., Kulkarni Y., Wright C., Kaushik V., O’Doherty G., Iyer A.K.V. Digitoxin and its Synthetic Analog MonoD have Potent Antiproliferative Effects on Lung Cancer Cells and Potentiate the Effects of Hydroxyurea and Paclitaxel. Oncology Reports (2016), 35(2), 878-86. 

    20.         Wright C. A., Kulkarni Y. M., Iyer A.K.V, Azad N.. S-nitrosylation of Bcl-2 Negatively Affects Autophagy in Lung Epithelial Cells. Journal of Cellular Biochemistry (2016), 117(2), 521-32. 

    21.         Yakisich J.S., Azad N., Venkatadri R., Kulkarni Y., Wright C., Kaushik V., Iyer A.K.V. Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model. Stem Cells International (2016).Volume 2016, Article ID 5603135, 6 pages (Open Access Article).URL: http://dx.doi.org/10.1155/2016/5603135

    22.         Kulkarni Y. M., Dutta S., Iyer A.K.V, Venkatadri R., Kaushik V., Ramesh V., Wright C. A., Semmes O.J., Yakisich J.S., Azad N.. A Proteomics Approach to Identifying Key Protein Targets Involved in VEGF-Inhibitor Mediated Attenuation of Bleomycin-Induced Pulmonary Fibrosis. Proteomics (2016), 16(1), 33-46.

    23.         Iyer A.K.V, Ramesh V., Castro C.A., Kaushik V., Kulkarni Y. M., Wright C. A., Venkatadri R., Rojanasakul Y., Azad N.. Nitric Oxide Mediates Bleomycin-Induced Angiogenesis and Pulmonary Fibrosis via Regulation of VEGF. Journal of Cellular Biochemistry (2015), 116(11), 2484-93.

    24.         Kulkarni Y., Kaushik V., Azad N., Wright C., Rojanasakul Y., O’Doherty G., Iyer A.K.V.  Autophagy-Induced Apoptosis in Lung Cancer cells by a Novel Digitoxin Analog. Journal of Cellular Physiology (2015) (Epub ahead of Print)

    25.         Talbott S.J., Luanpitpong S., Stehlik C., Azad N., Iyer A.K.V., Wang L., Rojanasakul Y. S-Nitrosylation Of FLICE Inhibitory Protein Determines Its Interaction With RIP1 And Activation Of NF-Κb. Cell Cycle (2014), 13(12), 948-957.

    26.         Iyer, A.K.V, Azad N., Rojanasakul, Y. Nitrosothiol Signaling and Protein Nitrosation in Cell Death and Survival. Nitric Oxide Biology and Chemistry (2014), 42C, 9-18.

    27.         Azad N., Iyer A. K. V. Reactive Oxygen Species and Apoptosis. Systems Biology of Free Radicals and Antioxidants (2014), 113-135.

    28.         Azad N., Iyer A.K.V., Lu Y., Wang L., Rojanasakul Y. P38/MAPK Regulates Single-walled Carbon Nanotube-Induced Fibroblast Proliferation and Collagen Production. Nanotoxicology (2013),7(2), 157–168.

    29.         Luanpitpong S., Iyer A.K.V., Azad N., Wang L., Rojanasakul Y. Nitrosothiol Signaling in Anoikis Resistance and Cancer Metastasis. Forum on Immunopathological Diseases and Therapeutics (2012),3(2), 141–154.

    30.         Djordje M., Luanpitpong S., Azad N., Wang L., Jiang B. H., Davis M., Barnett J., Guo L., Rojanasakul Y. Multifunctional Role of Bcl-2 in Malignant Transformation and Tumorigenesis of Cr(VI)-Transformed Lung Cells. PLoS ONE (2012), 7(5), e37045.

    31.      Iyer, A.K.V, Azad N., Rojanasakul Y, Ma J.K.H. Application of Nanosciences in Pharmaceutics. Basic Physical Pharmacy (2012), 486-516.

    32.         Iyer A.K.V., Azad N., Wang L., and Rojanasakul Y. Cellular FLICE-Inhibitory Protein Regulates Fas-Ligand Induced Activation of NFκB in A PI3K-Dependent Manner. Journal of Immunology (2011), 187(6), 3256-66.

    33.         Iyer A.K.V., Zhou M., Azad N., Elbaz, H., Wang H., Rogalsky D., Rojanasakul Y., O’Doherty G. and Langenhan J. A Direct Comparison of the Anticancer Activities of Digitoxin MeON-Neoglycosides and O-Glycosides: Oligosaccharide Chain Length Dependant Induction of Caspase-9-Mediated Apoptosis. American Chemical Society Medicinal Chemistry Letters (2010), 1(7), 300-369.

    34.         Azad N., Iyer A.K.V., Wang L., Lu Y., Medan D., Castranova V., Rojanasakul Y. Nitric Oxide-Mediated Bcl-2 Stabilization Potentiates Malignant Transformation of Human Lung Epithelial Cells. American Journal of Respiratory Cellular and Molecular Biology (2010), 42 (5), 578-585.

    35.         Azad N., Lu Y., Wang L., Iyer A.K.V., Castranova V., Jiang B.H., Rojanasakul Y. Phosphatidylinositol-3-Kinase/Akt Regulates Bleomycin-Induced Fibroblast Proliferation and Collagen Production. American Journal of Respiratory Cellular and Molecular Biology (2010), 42 (4), 432-441.

    36.         Azad N., Iyer A.K.V., Vallyathan V., Wang L., Castranova V., Stehlik C., Rojanasakul Y. Role of Oxidative/Nitrosative Stress-Mediated Bcl-2 Regulation in Apoptosis and Malignant Transformation. Annals of The New York Academy of Sciences (2010), 1203, 1-6.

    37.      Iyer, A.K.V, Azad N., Rojanasakul, Y. S-nitrosylation – How Cancer Cells Say NO to Cell Death. Nitric Oxide Biology and Chemistry, Prognosis, Prevention and Therapy (2010), Part 3, 4-102.

    38.      Azad N., Iyer A. K. V., Rojanasakul Y. Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis. Methods in Molecular Biology (2009), 559, 117-130.

    39.         Azad N., Iyer A.K.V., Manosroi A., Wang L., Rojanasakul Y. Superoxide-Mediated Proteasomal Degradation of Bcl-2 Determines Cell Susceptibility to Cr(VI)-Induced Apoptosis. Carcinogenesis (2008), 29(8), 1538-1545.

    40.         Iyer, A.K.V, Azad N., Rojanasakul, Y. Role of S-nitrosylation in Apoptosis Resistance and Malignant Transformation. Nitric Oxide Biology and Chemistry (2008), 19(2), 146-151.

    41.         Kongkaneramit L., Sarisuta N., Azad N., Lu Y., Iyer A.K.V., Wang L., Rojanasakul Y. Dependence of Reactive Oxygen Species and FLIP on Lipofectamine-Induced Apoptosis in Human Lung Epithelial Cells. Journal of Pharmacology and Experimental Therapeutics (2008), 325(3), 969-977.

    42.         Wang L., Azad N., Kongkaneramit L., Chen F., Lu Y., Jiang B.H., Rojanasakul Y. The Fas Death Signaling Pathway Connecting Reactive Oxygen Species Generation and FLICE Inhibitory Protein Downregulation. Journal of Immunology (2008), 180(5), 3072-3080.

    43.         Azad N., Rojanasakul, Y. and Vallyathan, V. Inflammation and Lung Cancer: Roles of Reactive Oxygen/Nitrogen Species. Journal of Toxicology and Environmental Health, Part B (2008), 11(1), 1-15.

    44.      Azad N., Iyer A. K. V., Rojanasakul Y. DNA Microarrays in Drug Discovery and Development. Biopharmaceutical Drug Design and Development (2007), 2nd Edition, 47-66.

    45.      Azad N., Rojanasakul Y. Macromolecular Drug Delivery. Biopharmaceutical Drug Design and Development (2007), 2nd Edition, 293-324.

    46.         Moungjaroen J., Nimmannit U., Callery P.S., Wang L., Azad N., Lipipun V., Chanvorachote P., Rojanasakul Y. Reactive Oxygen Species Mediate Caspase Activation and Apoptosis Induced by Lipoic Acid in Human Lung Epithelial Cancer Cells through Bcl-2 Downregulation. Journal of Pharmacology and Experimental Therapeutics (2006), 319(3), 1062-1069.

    47.         Azad N., Vallyathan V., Wang L., Tantishaiyakul V., Stehlik C., Leonard S.S., Rojanasakul Y. S-nitrosylation of Bcl-2 inhibits its ubiquitin-proteasomal degradation: A novel anti-apoptotic mechanism that suppresses apoptosis. The Journal of Biological Chemistry (2006), 281(45), 34124-34134.

    48.         Azad N., Rojanasakul Y. Nanobiotechnology in Drug Delivery. American Journal of Drug Delivery (2006), 4(2), 79-88.

    49.         Azad N., Rojanasakul Y. Vaccine Delivery - Current Trends and Future. Current Drug Delivery (2006), 3(2), 137-146.

    50.         Chanvorachote P., Nimmannit U., Wang L., Stehlik C., Lu B., Azad N., Rojanasakul Y. Nitric Oxide Negatively Regulates Fas CD95-induced Apoptosis through Inhibition of Ubiquitin-Proteasome mediated Degradation of FLICE Inhibitory Protein. The Journal of Biological Chemistry (2005), 280(51), 42044–42050.

 

Selected Presentations

  • Venkatadri R., Wright C., Kaushik V., Kulkarni Y., Yakisich J.S., Iyer A.K.V, Neelam Azad. MnTBAP Inhibits Bleomycin-Induced Pulmonary Fibrosis by Regulating Canonical Wnt Pathway. PFF Summit: From Bench to Bedside, Pulmonary Fibrosis Foundation Annual Conference, 2015.(Poster)
  • Wright C., Iyer A.K.V, Neelam Azad. Differential autophagy responses in non-tumorigenic and malignantly transformed lung epithelial cells. AACR Annual Conference,2014.(Poster)
  • Neelam Azad, Rojanasakul Y., O’ Doherty G., Iyer A.K.V. Anti-tumorigenic Effects of a Novel Digitoxin Analogue. AACR Annual Conference, 2013. (Poster)
  • Neelam Azad. Role of Oxidative Stress Mediated Angiogenesis in Pulmonary Fibrosis. Graduate Seminar series, College of Pharmacy, Nova Southeastern University, 2012.(Podium)
  • Neelam Azad, Iyer A.K.V., Wang L., Y Lu., Rojanasakul Y. Impact of Oxidative Stress-Regulated Angiogenesis in Pulmonary Fibrosis. PFF Summit 2011: From Bench to Bedside, Inaugural Conference of Pulmonary Fibrosis Foundation, 2011. (Poster)
  • Neelam Azad. Molecular Mechanisms in Hexavalent Chromium-Induced Lung Pathogenesis. 6th Conference on Molecular Mechanisms of Metal Toxicity and Carcinogenesis. Sponsored by the National Institute for Environmental Health Sciences and Health and the University of Kentucky, 2010. (Podium)
  • Neelam Azad, Iyer A.K.V., Rojanasakul Y. Malignant Transformation of Human Lung Epithelial Cells – Interplay Between Nitric Oxide, Bcl-2 And VEGF. Towards Personalized Cancer Medicine, NYAS, 2010. (Poster)
  • Neelam Azad. Reactive Oxidative/Nitrosative Species in Lung Cancer and Fibrosis. 4th International Conference on Oxidative/Nitrosative Stress and Disease. The New York Academy of Sciences (NYAS), 2009. (Podium)
  • Neelam Azad, Iyer A.K.V., Lu Y., Wang L., Rojanasakul Y. Angiogenic Mediators Regulate Single-Walled Carbon Nanotube-Induced Fibrogenesis in Human Lung Fibroblasts. AAPS, 2008. (Poster)
  • Neelam Azad, Wang L., Rojanasakul Y. Reactive Oxygen Species Mediate Apoptosis in Human Lung Epithelial Cancer Cells through Ubiquitin-Mediated Bcl-2 Downregulation. AAPS, 2007. (Poster)
  • Neelam Azad. S-Nitrosylation of Bcl-2 - A Novel Anti-Apoptotic Mechanism. American Association of Pharmaceutical Scientists (AAPS), 2006. (Podium)

 

Professional Affiliations

  • Rho Chi Pharmacy Honor Society
  • Sigma Xi Research Honor Society
  • American Association of Cancer Research (AACR)
  • Minorities in Cancer Research (MICR)
  • American Association of Colleges of Pharmacy (AACP)